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Detection of the New Ehrlichia Species Closely Related to Ehrlichia ewingii from Haemaphysalis longicornis in Yonaguni Island, Okinawa, Japan OAK
MATSUMOTO, Kotaro; TAKEUCHI, Toshihiko; YOKOYAMA, Naoaki; KATAGIRI, Yoshito; OOSHIRO, Mamoru; ZAKIMI, Satoshi; Gaowa; KAWAMORI, Fumihiko; OHASHI, Norio; INOKUMA, Hisashi.
We collected a total of 206 Haemaphysalis longicornis ticks by flagging in pastures in Yonaguni Island, Okinawa, Japan, in April 2008. Four of the 206 tick DNA samples tested were positive in a polymerase chain reaction (PCR) screening for the 16SrRNA gene of Anaplasmataceae. Partial sequences of 4 PCR products were identical to each other. Longer sequences of the 16SrRNA gene were successfully determined in 2 of the 4 tick samples, and the obtained 1,392 bp and 1,300 bp sequences revealed high similarity to the 16SrRNA gene sequences of the validated Ehrlichia species, including Ehrlichia ewingii, E. chaffeensis, and E. canis (98.3–98.6%). We also sequenced 1,304 bp of the groEL gene from the 2 tick samples, and found that these had the highest similarity...
Palavras-chave: 16SrRNA; Ehrlichia; GroEL; Haemaphysalis longicornis.
Ano: 2011 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3581
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Molecular phylogeny and evolution of the cone snails (Gastropoda, Conoidea) ArchiMer
Puillandre, N.; Bouchet, P.; Duda, T. F., Jr.; Kauferstein, S.; Kohn, A. J.; Olivera, B. M.; Watkins, M.; Meyer, C..
We present a large-scale molecular phylogeny that includes 320 of the 761 recognized valid species of the cone snails (Conus), one of the most diverse groups of marine molluscs, based on three mitochondrial genes (COI, 16S rDNA and 12S rDNA). This is the first phylogeny of the taxon to employ concatenated sequences of several genes, and it includes more than twice as many species as the last published molecular phylogeny of the entire group nearly a decade ago. Most of the numerous molecular phylogenies published during the last 15 years are limited to rather small fractions of its species diversity. Bayesian and maximum likelihood analyses are mostly congruent and confirm the presence of three previously reported highly divergent lineages among cone...
Tipo: Text Palavras-chave: Ancestral state reconstruction; Conidae; Conus; COI; 16SrRNA; 12SrRNA.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00468/57920/83480.pdf
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Subseafloor archaeal communities: from the surface to a deep hot biosphere? ArchiMer
Roussel, Erwan.
The sub-seafloor biosphere may contain two thirds of Earth's total prokaryotic biomass. The large and active microbial populations buried in the sub-seafloor play a key role in global biogeochemical cycles. However, little is known about these prokaryotic communities. The depth limit of this sub-seafloor biosphere is still unreached, and elevated temperatures as well as insufficient energy sources are the likely factors limiting life at depth. Interestingly, archaeal communities with unknown physiologies and no cultured relatives seem to have a key role in deep marine sediments and hydrothermal ecosystems. As most deep biosphere microorganisms detected so far have been extremely resistant to cultivation, molecular approaches remain to date the most...
Tipo: Text Palavras-chave: Sediment; Hydrothermal system; Ultramafic; CM DGGE; DGGE; PmoA; DsrA; McrA; AmoA; 16SrRNA; Bacteria; Archaea; Deep biosphere; Sédiment; Écosystème hydrothermal; Ultramafique; CM DGGE; DGGE; PmoA; DsrA; McrA; AmoA; 16SrRNA; Bacteria; Archaea; Biosphère profonde.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/these-4630.pdf
Registros recuperados: 3
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